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Genoplante

CIRAD

South Green bioinformatics platform

Oryza Tag Line consists in a searchable database integrating phenotypic data resulting from the evaluation of the Génoplante rice insertion line library. To display the sequence information (Flanking Sequence Tags) resulting from the molecular characterization of the  mutagen insertion sites in the collection, Oryza Tag Line is linked to OryGenesDB a GGB-derived interface.
Aside from generic data ( production record and seed stock ) Oryza Tag Line includes textual and pictorial information resulting from the following observations: (i.) GUS/GFP expression assays conducted on the primary transformants (T0) and/or T1 progenies (ii.) morpho-physiological alterations detected during the growth of the T1 progenies either in phytotron -up to the 5-6 leaf stage- or under agronomical conditions in the field (collaboration with CIAT, Cali, Colombia).

Other phenotype information will be integrated in the future including: (i.) reporter gene expression in mature seed and changes in seed morphology detected during the systematic screening of the panicles harvested on T0 and T1 plants. (ii.) changes observed in the response to inoculation with Magnaporthe grisea.

For these observations, we use the controlled vocabulary from Plant Ontology and Gene Ontology.


Forward genetic search is possible through queries based on either phenotype alterations or reporter gene expression combined with a navigation by organ, developmental stage and trait category. Alternatively, search can also be conducted by keywords for a free navigation. We also propose to combine the different types of queries in an advanced search page. The Line ID search permits to display the detailed features of a given line or of several lines through pasting a list of ID numbers. The ontology ID search allows a search by Trait ontology

Access to FST information through OrygenesDB

We hope you will enjoy using this site ! Please feel free to contact us if you have any suggestions or questions
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Database Contents

lines
with T2 seed

21 588 entries

lines
with FST
23 364 entries

Expression
recorded

2 255 entries

Pictures

50 777 entries

Phenotypes
recorded

13 000 entries

FST in Genbank
42 443 entries
       
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