Figure 4: Southern analysis of T-DNA inserts in the Génoplante library of insertion lines
A: Distribution of the primary transformants as a function of the number of T-DNA copies integrated (left) and Southern blot analysis of Sst1 digests of DNA isolated from a set of primary transformants and hybridized to the hph (upper panel) and gusA (lower panel) probes (right).
B :Frequency of binary vector-free primary transformants as a function of the number of T-DNA copies integrated (left) and Southern blot analysis of Sst1 digests of DNA isolated from a set of primary transformants and hybridized to the binary vector probe (right). (Rouvière et al. Unpublished)